Varicella-Zoster Virus IE4 Protein Interacts with SR Proteins and Exports mRNAs through the TAP/NXF1 Pathway
Figure 8
Knockdown of TAP/NXF1 alters expression of some VZV proteins.
HEK293 cells were not transfected (lanes 1 and 4) or transfected with the negative siRNA pool (lanes 2 and 5) or the TAP/NXF1 siRNA pool (lanes 3 and 6) for 24 h followed by mock- (lanes 1 to 3) or VZV-infection for 36 h (lanes 4 to 6). Expression of the different proteins was verified by Western Blotting with anti-TAP/NXF1, anti-IE4, anti-IE63, anti-ORF29p, anti-ORF9p, anti-gE and anti-GAPDH (loading control) antibodies as indicated.