Stereotaxical Infusion of Rotenone: A Reliable Rodent Model for Parkinson's Disease
Figure 5
Double immunohistostaining of alpha-synuclein and TH.
Samples of the double immunohistostained alpha-synuclein and TH of the lesioned SNc were taken from the rats sacrificed four weeks after surgery (3-µg group: A–H; 12-µg group: I–L). Alpha-synuclein was visualized by FITC-conjugated donkey-anti-mouse IgG, TH was labeled by the Cy3-conjugated goat-anti-rabbit IgG, and the nucleus was stained by the Hoechst 33258. The last graph of the three rows was obtained by the overlapping the first three graphs (Scale bars = 50 µm). Compared with the contralateral side of the ST models (A-D), a dose-dependent increase in alpha-synuclein expression in the lesioned brain and a decrease in the number of TH-positive neurons are shown in E–H (3-µg group) and I–L (12-µg group). The quantitative data were shown as Figure 4L.