Selective Inhibition of Retinal Angiogenesis by Targeting PI3 Kinase
Figure 5
LY294002 attenuates ectopic developmental angiogenesis in obd mutants.
Wildtype Tg(fli1:EGFP) larvae (wt) or out of bounds mutants in the Tg(fli1:EGFP) background (obd) were treated with 10 µM LY294002 from 1–5 dpf before dissecting lenses and analysing hyaloid vasculature development. LY294002 inhibits normal developmental angiogenesis in wildtype Tg(fli1:EGFP) larvae as seen by the reduced vessel patterning in lateral views of the lens (A, D). LY294002 also inhibits the extraneous hyaloid branch patterning (B) and branch number (C) observed in obd mutants as observed by reduction of branch patterning to near normal levels (E and F). Arrows depict the orientation of the lens, pointing in the direction from the optic disk to lens. G) Quantification of the reduction in the extraneous primary hyaloid branches number in LY294002-treated obd mutants. The data is plotted as the average and standard deviation of larvae (obd−/− n = 7, obd−/− treated: n = 8) from 2 independent experiments (***p<0.001, t-test). OD: Optic Disc.