RNA Aptamers Generated against Oligomeric Aβ40 Recognize Common Amyloid Aptatopes with Low Specificity but High Sensitivity
Figure 6
Reactivity of the 3rd and the 6th RNA pools with Aβ40 and other amyloid fibrils.
(A) Six SELEX cycles were performed using cross-linked LMW Aβ40 and the progression of SELEX was assessed after two counter-selection cycles by testing the reactivity of the RNA pool for Aβ40 fibrils (400 and 800 ng) or cross-linked Aβ40 (1,000 ng). Blots were probed with RNA pools generated before (3rd cycle) and after (6th cycle) counter-selection. (B) Fibrillar preparations of Aβ40, Aβ42, calcitonin, IAPP, insulin, lysozyme, and prion106–126 were spotted at 5, 50, and 500 ng and the membranes were probed with the 6th RNA pool.