IL-3 and Oncogenic Abl Regulate the Myeloblast Transcriptome by Altering mRNA Stability
Figure 2
IL-3 regulated turnover of transcripts.
A–D. Northern Blots of IL-3 stabilized transcripts. Northern blots of samples from one (D) or two independent experiments each (A, B, C). RNAs were prepared from 32Dcl3 cells washed free of IL-3, transcriptionally arrested and then exposed to medium alone (−) or reexposed to 1 ng/ml IL-3 (+) for indicated times. Blots were probed with riboprobes prepared for transcripts selected from microarray-based rank listings. GAPDH expression is shown as a loading control. p21 is probed as a positive control in each of the 7 experiments. For each time point, lower expression of the sample lacking IL-3 (−) is evident. E. left: Graphic representation of Northern blot results. Decay curves are plotted from the data in A–D quantitated with Image J software. *p< = 0.05, **p<0.002 comparison of value with and without IL3. right: Transcript half-lives calculated as described in Methods for IL-3 positive and -negative conditions.