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Optimizing Taq Polymerase Concentration for Improved Signal-to-Noise in the Broad Range Detection of Low Abundance Bacteria

Figure 1

Detection of Pseudomonas fluorescens at high and low Taq polymerase concentrations.

Bacterial detection with 0.5 Units (A) or 0.05 Units (B) Qiagen Taq DNA polymerase with 16S350B assay on samples containing 103, 102, 101 and zero Pseudomonas fluorescens bacteria. A composite of A and B is shown in C.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0007010.g001