Caspase-12 and the Inflammatory Response to Yersinia pestis
Figure 2
In-vitro cytokine measurements after whole blood LPS and Pam3Cys stimulation between the caspase-12 genotypes from the volunteers enrolled in 2007.
Stimulation with LPS was performed with 10 ng/ml. TNF (A), IL-1β (B) and IL-10 (C) were measured by ELISA after 24-hour stimulation. Volunteers were grouped as homozygous bearing the nonfunctional caspase-12 genotype (S/S), heterozygous bearing the nonfunctional and functional caspase-12 (S/L) and homozygous that bear the functional caspase-12 (L/L). Numbers of volunteers included for each cytokine are S/S = 24, S/L = 22 and L/L = 1. Values represent mean + SD for each group of volunteers. P-values for differences between caspase-12 genotype were calculated with the Mann-Whitney test.