Identification of Keratinocyte Growth Factor as a Target of microRNA-155 in Lung Fibroblasts: Implication in Epithelial-Mesenchymal Interactions
Figure 8
miR-155 overexpression or knock-down regulates KGF release in TNF-α- or IL-1β stimulated human lung fibroblasts.
Panels A-B show a representative experiment of pre-miR-Neg/pre-miR-155 (10 nM) or LNA-159s/LNA-155 (25 nM) transfection effect on KGF release by lung fibroblast stimulated or not during 48 hours with 10 ng/ml TNF-α or IL-1β. Results correspond to triplicates measurements of KGF by ELISA (R&D System). *: p-value<0.05; **: p-value<0.01. Panel C: model of miR-155-dependent attenuation of KGF release following fibroblasts stimulation with inflammatory cytokines.