Epigenetic Engineering of Ribosomal RNA Genes Enhances Protein Production
Figure 1
a. qRT-PCR of TIP5 mRNA of NIH/3T3 cells stably expressing shRNA-TIP5-1 and TIP5-2 sequences and of HEK293T cells stably expressing miRNA-TIP5-1 and TIP5-2 sequences. Data were normalized to GAPDH mRNA levels. Data are from two independent experiments. b. Semiquantitative RT-PCR of TIP5 mRNA of stable shRNA-TIP5-1/2 NIH/3T3, miRNA-TIP5-1/2 HEK293T and miRNA-TIP5-1/2 CHO-K1 cells. As control, RT-PCR of GAPDH mRNA is shown. Data are from two independent experiments. c. TIP5-specific Western blot analysis of stable shRNA-TIP5-1/2 NIH/3T3 and miRNA-TIP5-1/2 HEK293T cells. CHO-K1 cells could not be analyzed because the anti-TIP5 antibody does not recognize hamster TIP5. To normalize protein loading, the levels of UBF were monitored using an anti-UBF antibody.