Genome-Wide mRNA Expression Analysis of Hepatic Adaptation to High-Fat Diets Reveals Switch from an Inflammatory to Steatotic Transcriptional Program
Figure 3
Temporal modules of pathway activities during the hepatic high-fat response.
Hierarchical clustering (Pearson correlation (uncentered), average linkage) of Normalized Enrichment Scores (NES), as determined by Gene Set Enrichment Analysis. The NES values of 314 gene sets, significant (FDR q-value<0.1) in at least one of HFBT vs. chow and HFP vs. chow per time-point comparisons are used as an input for hierarchical clustering. The NES scores, represented by the color gradient, correspond to the relative up- (red) and down- (blue) regulation of the gene sets under each of experimental conditions. The cluster diagram can be divided into five main temporal themes (depicted as modules 1 to 5), highlighting the main trends in temporal pathway activities: (1) early activation/late repression; (2) constant repression; (4) constant activation and (5) early repression/mid and late activation. Module (3) includes fuzzy pathway profiles that bring to light differences in transcription response to beef tallow- (HFBT) and palm oil-based (HFP) high-fat diets.