Resolving Cell Population Heterogeneity: Real-Time PCR for Simultaneous Multiplexed Gene Detection in Multiple Single-Cell Samples
Figure 6
Distribution of cytokine protein expression in single cells.
BMDMs were not stimulated (dotted line) or stimulated with for 4 hours in the presence of brefeldin A. The abundance of TNF and IL1β were detected by 2-color immunofluorescence and flow cytometry. The histogram shows the distribution of expression of the indicated cytokines in unstimulated cells (dotted line) and LPS (thick solid line) and poly I∶C (thin solid line) stimulated cells.