Drug and Cell Type-Specific Regulation of Genes with Different Classes of Estrogen Receptor β-Selective Agonists
Figure 3
Time-course of gene regulation by E2 and ERβ-agonists in U2OS cells.
U2OS-ERα or U2OS-ERβ cells grown in stripped fetal bovine serum were untreated or treated with doxycycline for 18 hours to induce ERs. The cells were then treated with E2 (10 nM), LIQ (1 µM), NYA(1 µM), DPN (1 µM), ERB-041(1 µM), and MF101 (125 µg/ml). Following treatment, mRNA levels for AKAP1, IL-17, and K19 were measured at 1, 3, 6 and 12 hours by real-time PCR in U2OS-ERα (A, C, and E, respectively) and U2OS ERβ (B, D, and F, respectively) cells. Each data point is the average of triplicate determinations. Error bars represent the mean±S.E.M.