Nucleocytoplasmic Shuttling of the TACC Protein Mia1p/Alp7p Is Required for Remodeling of Microtubule Arrays during the Cell Cycle
Figure 1
Mia1p is exported from the nucleus via a Crm1p-dependent NES.
(A) Mia1p-GFP localized to mitotic spindles and interphase microtubules in crm1-809 Uch2p-mCherry expressing cells at 36°C but only to the nucleus at 18°C. (B) Positions of predicted NLS and NES on Mia1p. (C) Mia1p-ΔC17-GFP is enriched in the nucleus in interphase cells. (D) GFP-NES but not MutNES is excluded from the nucleus. Shown are single maximum intensity reconstructions of live cells. Scale bars = 5 µm.