Xeno-Free Defined Conditions for Culture of Human Embryonic Stem Cells, Neural Stem Cells and Dopaminergic Neurons Derived from Them
Figure 1
Generation of NSCs from hESCs adapted to defined medium.
hESC line I6 at passage 42 was adapted to a chemically defined medium StemPro. (A–B) Morphology (A) and expression of the pluripotent marker Oct4 (B) in hESCs that were cultured in StemPro for 28 passages. (C–F) Generation of NSCs in defined conditions. Nestin+ neural tube-like rosette structures were formed in the center of the ESC colonies after 12–14 days of differentiation (C–D). A monolayer of homogeneous NSCs expressed Sox1 and nestin (E–F).