Exercise-Training in Young Drosophila melanogaster Reduces Age-Related Decline in Mobility and Cardiac Performance
Figure 6
Mito-efficient flies display improved mobility.
(A): Negative geotaxis of aging, exercise-trained, La flies. Ra exercise-trained flies (green triangles) display improvement in climbing index at day 7 of the exercise-training regimen and this difference persists two weeks after cessation of exercise-training compared to the unexercised controls (purple Xs) (multivariate regression, treatment, p<0.0001; treatment-by-age: p = 0.0023). In contrast, La flies show a slower age-related decline in mobility compared to Ra flies (La exercise-trained (blue diamonds) versus Ra exercise-trained (green triangles): (multivariate regression, genotype-by-age: p<0.0001). (B): Activity rate is measured as number of times crossing a beam threshold per 10 minutes during 2-hour intervals. y1w67c23 exercise-trained flies (blue diamonds) have higher activity rates over 4 weeks compared to y1w67c23 unexercised flies (red squares) (multivariate regression, treatment-by-age: p = 0.0365) and y1w67c23 control flies (green triangles) not placed on the Power Tower. Unexercised flies placed on the machine and control flies not placed on the machine are not significantly different from each other (multivariate regression, treatment-by-age: p = 0.2237). La flies (purple Xs) not placed on the machine have a much higher activity rate throughout 4 weeks compared to the wildtype. (C): Arrest rate for La exercise-trained flies (blue diamonds) is not significantly different from La unexercised flies (red squares) across 5 weeks (multivariate regression, treatment: p = 0.8688, treatment-by-age: p = 0.3784).