Regulation of Serum Amyloid A3 (SAA3) in Mouse Colonic Epithelium and Adipose Tissue by the Intestinal Microbiota
Figure 6
LPS induces SAA3 and TNF-α mRNA expression in CMT-93 colonic epithelial cells and RAW 264.7 macrophages.
(A) qRT-PCR analysis of SAA3 mRNA expression levels in CMT-93 cells and RAW 264.7 macrophages after 1 h LPS at the indicated concentrations. (B) qRT-PCR analysis of TNF-α mRNA expression levels in CMT-93 cells and RAW 264.7 macrophages after 1 h LPS at the indicated concentrations. (C) SAA3 mRNA expression levels in CMT-93 cells and RAW 264.7 macrophages after 1 h treatment with recombinant TNF-α (10 ng/ml). n = 6 biological replicates per treatment. *P<0.05, **P<0.01, ***P<0.001; Student's t-test.