Regulation of Serum Amyloid A3 (SAA3) in Mouse Colonic Epithelium and Adipose Tissue by the Intestinal Microbiota
Figure 5
SAA3 is expressed in both colonic epithelial cells and intraepithelial macrophages.
(A) Multilabel immunohistochemistry revealed that multiple cells in the CONV-R mouse colonic epithelium express SAA3 (green). (B) Presence of F4/80-positive cells (red; pan-macrophage marker) by intraepithelial macrophages. (C) Arrowheads point to four intraepithelial macrophages (positive for SAA3 and F4/80) at the luminal interface with bacteria. Nuclei are stained with bis-benzimide (blue). (D) Representative staining with primary antibodies omitted. Scale bars = 30 µm.