Abnormalities in Osteoclastogenesis and Decreased Tumorigenesis in Mice Deficient for Ovarian Cancer G Protein-Coupled Receptor 1
Figure 6
Reduction of TG-induced peritoneal macrophage numbers and NO production in OGR1 KO mice in the mixed background.
(A) The percentages of monocytes in peripheral blood were higher in KO than that in WT mice. Blood samples were obtained from facial vein of FL and KO mice (n = 6 in each group). (B) TG-stimulated peritoneal macrophage numbers in KO mice were reduced when compared to those from WT mice (n = 20 in each group). (C) Bone marrow-derived macrophage numbers in FL and KO mice after M-CSF stimulation (n = 6 in each group) were not significantly different. (D) Nitric Oxide production from KO peritoneal macrophages was reduced than that of WT macrophages. Macrophages were stimulated with LPS (330 ng/mL) for 18 hr. (E) LPS-induced ERK activation was reduced in OGR1 KO macrophages. TG-induced peritoneal macrophages were treated with LPS (100 µg/mL) (a), or LPC (2.5 µM) (b) for 30 min prior to sample collection for Western blot analyses. (F) pH-modulated cAMP formation (a) or cell survival (b) was not significantly different in peritoneal macrophages from FL and KO mice. Cells were treated with indicated pH buffer and IBMX (1 mM) for 30 min. Proton effect on the survival of macrophages were conducted for 20 hr. The results in this figure are representative of at least 3 independent experiments. * represents a P value of <0.05, and *** represents a P value of <0.001.