Intra-Platform Repeatability and Inter-Platform Comparability of MicroRNA Microarray Technology
Figure 2
Intra-platform repeatability of quantitative assessment of microRNA expression.
MicroRNA measurement of the same sample (L: human liver, P: human prostate) was replicated three times. Only data of microRNAs with positive detection call were used for analysis. 2A: Scatter plots show the correlation between replicate 1 and 2 (scatter plots for all possible combinations between three replicates were shown in Figure S4). Spearman's correlation coefficients (Rs) for replicates 1 vs. 2, 1 vs. 3, and 2 vs. 3 were calculated and summarized in lower table. Rs for the prostate sample were generally better than those for the liver sample (p = 0.0005, paired T-test). This finding suggests that repeatability of microRNA would depend on the sample cell type, and that repeatability in the case of samples expressing A higher amount of microRNAs would be better. TAQ (Taqman analysis) obtained the best Rs values despite a slightly wider spread of data. It might be a result from wider range of microRNA detection (microarray: 216, Taqman: about 220). 2B: Box plot of coefficient of variation (CV) for microRNA detection platforms. The coefficient of variation for each microRNA assessment was calculated by a formula, CV = (standard deviation/mean)×100, and the distribution of CV was plotted in the box plot diagram. Bold line: median, bottom and top line of the box: first and third quantile, respectively.