Innervation of Gonadotropin-Releasing Hormone Neurons by Peptidergic Neurons Conveying Circadian or Energy Balance Information in the Mouse
Figure 5
A: MCH-ir perikarya localized in the lateral hypothalamic area (A). Examples of close appositions between MCH-ir fibers (red) and GnRH-GFP perikarya and processes (green) in a maximum intensity projection of the image stack (B) and in single 0.5 µm optical slices (C, D). MCH -ir fibers and GnRH-ir terminals were observed in proximity in the median eminence (E), where the presence of close appositions was confirmed by analysis of single 0.5 µm optical slices (F). Scale bars = 25 µm (A), 5 µm (B–D), 10 µm (E), 5 µm (F).