FRAP Analysis on Red Alga Reveals the Fluorescence Recovery Is Ascribed to Intrinsic Photoprocesses of Phycobilisomes than Large-Scale Diffusion
Figure 4
Selected analysis of the fluorescence profiles across the edge of the bleached area in partially-bleached cells, allowing the real-time exploration of the fluorescence dynamics of PBsomes correlated to their lateral diffusion.
A, untreated cell; B, betaine-treated cell; C, glutaraldehyde-treated cell. Area (1), the unbleached cellular area; Area (2), the slope of the fluorescence intensity gradient; Area (3), the fluorescence recovery as a function of distance from the edge of the bleached area. The mean fluorescence intensities of three different regions (R1, R2 and R3 in Figure 4A–C) at distinct recovery time were analyzed: D, from untreated cell; E, from betaine-treated cell; F, from glutaraldehyde-treated cell. Fluorescence intensities are normalized at zero second. As shown, no remarkable difference of the fluorescence intensities in these three cellular areas could be observed, indicating the fluorescence recovery of PBsomes does not differ as a function of distance from the edge of the bleached area of theses cells.