Further Characterisation of the Molecular Signature of Quiescent and Activated Mouse Muscle Satellite Cells
Figure 3
Jagged-1 is induced when satellite cells are activated.
EDL myofibres were cultured in suspension for 48 hrs in plating medium to elicit the activation and proliferation of satellite cells (arrows). Activated and proliferating satellite cells were identified by immunostaining for MyoD (green), and Caveolin-1 (a - red), integrin α7 (b - red) and CTR (c - red) remained expressed in the plasma membrane of activated satellite cells. The continued presence of both emerin (red, d) and lamin A/C (green, e) in the nuclear envelope of activated satellite cells expressing MyoD (green in d, red in e) was also noted. Moreover, we found that the Notch ligand Jagged-1 (red) was induced upon activation, in MyoD+ve (green) satellite cells (f). Nuclei were counterstained with DAPI (blue). Scale bar represents 20 µm.