A Cytokine–Cytokine Interaction in the Assembly of Higher-Order Structure and Activation of the Interleukine-3:Receptor Complex
Figure 3
A cytokine–cytokine interaction interface in the dodecamer.
(A) A ribbon/surface presentation of interface IV and V (dashed boxes) in the modeled IL-3:receptor complex. Only domain 4 of the interaction beta subunit and IL-3 are shown in this view. The two residues at interface IV (Glu359 and Lys362) that engage in potential charge repulsion are indicated. The arrows indicate the two fold symmetry axis that runs through both interface IV and V. (B) A top view of interface V in the crystal structure of the GM-CSF:receptor complex (dashed box) and its relative position with respect to other components of the complex. The receptor alpha subunit domain 1 showed only partial density of a few beta strands that are represented by backbone trace here. (C) A top view of interface V (dashed box) mediated by the AA′ loop in the modeled IL-3:receptor complex.