Epitope Characterization and Variable Region Sequence of F1-40, a High-Affinity Monoclonal Antibody to Botulinum Neurotoxin Type A (Hall Strain)
Figure 5
Competition ELISA for F1-40 by Lc peptide fragment mutants in solution.
The percent inhibition of binding of mAb F1-40 to immobilized Lc peptide fragment on a 96-well plate by various peptide fragments in solution (♦, Lc; ▵ Lc-Δ; ○ Lc-QPD; ▪ Lc-RS) was calculated by the formula (1-B/B0)×100, where B = luminescent counts at each concentration of fusion peptide in solution, and B0 = luminescent counts at 0 µg/mL fusion peptide in solution. Data is presented±1 standard error, n = 4.