The Role of p300 Histone Acetyltransferase in UV-Induced Histone Modifications and MMP-1 Gene Transcription
Figure 5
p300HAT mediates UV-induced p53 expression and histone modification.
A and B, U2OS (p53wt) and SAOS-2 (p53−/−) cells were UV-irradiated and post-treated with 7.5 µM AA for 1 h. γ-H2AX, p53, and acetyl-H3 in total cell lysates were analyzed by western blotting (upper panels). Bar graphs (lower panels) show quantitative analysis of scanning densitometric values of γ-H2AX, p53 and acetyl-H3 as ratios to β-actin and histone H3 expression, which were used as loading controls. C: control, UV: UV-irradiated cells, AA: AA treated cells, UV/AA: UV irradiated cells incubated with AA. Values represent the mean±SEM of data from three independent experiments. *P<0.05, **P<0.01 vs. control; † P<0.05, † † P<0.01 vs. UV.