Two-Dimensional Electrophoresis of Tau Mutants Reveals Specific Phosphorylation Pattern Likely Linked to Early Tau Conformational Changes
Figure 1
Analysis of transgenes expression in 4RTau cell lines.
A) Lysates from Mock and 4RTau cells, treated or not (0) with tetracycline for 24 to 48 hours were immunolabeled with TauCter 1902 (Total Tau), and β-actin as loading control. B) Quantification of Tau expression levels in 4RTau cell lines: Ratios of densitometric values of TauCter1902/β-actin immunoreactivities are presented. Ratios are normalized to those obtained from WT 4RTau cells after 48 hours tetracycline (arbitrary value = 1).