Auto-Ubiquitination-Induced Degradation of MALT1-API2 Prevents BCL10 Destabilization in t(11;18)(q21;q21)-Positive MALT Lymphoma
Figure 6
MALT1-API2 reduces NF-κB activation by API2-MALT1.
NF-κB-dependent luciferase assay after co-expression of API2-MALT1 (A7M3/A7M5/A7M8) with its corresponding MALT1-API2 fusion variant (M2A8/M4A8/M7A8) in HEK-293T. NF-κB-dependent luciferase activity is represented as fold induction of vector-transfected cells. Shown is one representative experiment of 2, performed in triplicate, with mean and standard deviation. Lysates were immunodetected with α-Flag to confirm comparable API2-MALT1 expression in all cases.