Identifying Critical Non-Catalytic Residues that Modulate Protein Kinase A Activity
Figure 5
Suppressor mutations from the prkaca-F327A background.
A, Highlighted suppressor mutations identified from the prkaca-F327A background. The original F327A mutation is highlighted in yellow, and suppressor mutations are shown in blue. PKA features are highlighted as follows: small lobe, gray; large lobe, sand; C-terminal tail,red; PKI: magenta. Some frequently highlighted mutations are labeled. The image was made using PDB file 1ATP and modeled using PyMol. B, Mutations isolated from the same suppressor are connected by a dashed line. Single suppressor mutations are listed above the corresponding secondary structural elements of PKA. Secondary structural elements are represented where alpha-helices are red and beta-sheets are blue.