The Cellular Distribution of Serotonin Transporter Is Impeded on Serotonin-Altered Vimentin Network
Figure 4
Vimentin binding domain on the C-terminus of SERT.
CHO cells expressing the indicated SERT constructs were lysed and prepared for IP. The cell lysate was incubated with monoclonal anti vimentin-Ab coated protein A sepharose beads. The presence of SERT truncations was detected with a polyclonal SERT Ab (Chemicon International). Since SERT-Δ6 bound to vimentin and the other truncations, we proposed that the amino acids 616–624 are at least one of the vimentin binding domains on the C-terminus of SERT. Nonspecific adsorption of Sepharose beads was not determined in the absence of vimentin-Ab and the data obtained from this set of experiment is not presented here since it can be found in Figure 1B. All lanes contain protein recovered from the same number of cells equivalent to 30% of one well from a confluent 24-well culture plate. Figure shows representative images from 3 separate experiments.