Conserved Protective Mechanisms in Radiation and Genetically Attenuated uis3(-) and uis4(-) Plasmodium Sporozoites
Figure 2
CS is a powerful protective T cell antigen in both P. yoelli RAS and uis3(-), uis4(-) GAPs.
BALB/c CS-Tg JhT (−/−) mice were primed and boosted with 1×105 P. yoelli RAS or with uis3(-) or with uis4(-) GAPs. All immunized mice were challenged with 1×104 wild type infectious sporozoites and infected livers were isolated 42 hours post infection (A) Liver stage burden in indicated groups of immunized mice were assessed by measuring the parasite specific 18S rRNA copy numbers by q-RT PCR. The results are expressed as mean±s.d of 18S rRNA copy numbers from 5 mice per group. (B) IFN-gamma ELISPOT assay to quantify CS specific T cells from indicated groups of JhT (−/−) immunized mice. Results are expressed as mean±s.d of CS-specific CD8+ T cells obtained from 5 immunized mice per group.