IL-10 Is Critically Involved in Mycobacterial HSP70 Induced Suppression of Proteoglycan-Induced Arthritis
Figure 5
Increased IL-10 mRNA expression in CD4+CD25+ cells of HSP70 immunized mice.
Mice were PBS or heat shock protein 70 (HSP70) immunized before induction of arthritis as described in figure 1. On day 38, CD4+CD25+ cells were isolated from the spleen followed by isolation of mRNA. Foxp3 and IL-10 mRNA expression was determined by Quantitative Real-Time PCR analysis. Data are normalized and expressed relative to HPRT and depicted as mean±SEM (n = 5 mice in each group). Significant difference (p<0.05, Mann-Whitney U Test) is indicated by the asterisk.