Porphyrin Homeostasis Maintained by ABCG2 Regulates Self-Renewal of Embryonic Stem Cells
Figure 6
Inhibition of ABCG2 resulted in increases in levels of γH2AX, p53, phosphorylated p53 and phosphylated ATM.
(A). RT-PCR data for genes involved in oxidative stress defense: SOD2, SOD4, TRX, and GPX2, with GAPDH as internal control for untreated and 10 µM FTC-treated mES-R1 cells 72 hours after addition of FTC. (B) Western blot of 10 µM FTC-treated mES-J1 cells stained for Nanog, γH2AX, p53 and Phospho-p53 Ser18, 23 and 389 as well as ATM and Phospho-ATM ser1981. Equal amounts of protein were used (40 µg per lane). (C–F) Immunofluorescence staining for γH2AX (C, D) and p53 (E, F) in untreated and 10 µM FTC treated mES-R1 cells taken at 72 hours after addition of FTC.