Stat3 and c-Myc Genome-Wide Promoter Occupancy in Embryonic Stem Cells
Figure 3
Q-PCR analysis of Stat3 and c-Myc ChIP-enriched regions.
(A) Representative Stat3 genomic binding sites confirmed by Q-PCR. Red stars denote ChIP-chip enriched DNA sequences that were amplified using Q-PCR. (B) Confirmation of Stat3-ChIP enriched TF binding sites by Q-PCR. (C) Representative c-Myc genomic binding sites confirmed by Q-PCR. Blue stars denote ChIP-chip enriched DNA sequences that were amplified using Q-PCR. (D) Confirmation of c-Myc-ChIP enriched TF binding sites by Q-PCR. Q-PCR was performed on non-amplified Stat3-, c-Myc-, and IgG-ChIP enriched and Input DNA. Results are shown as percent enrichment relative to Input. MA enrichment values adjusted to log2 are shown in addition to sequence position. Stat3 and c-Myc enrichment are shown as red and blue heat maps respectively, while conservation is shown as a grayscale heat map.