Tubulin Dimers Oligomerize before Their Incorporation into Microtubules
Figure 6
Microtubule nucleation intermediates.
Images were taken at successive time points from preparations containing 2.5 µM tubulin, 1 mM GMPCPP, 10% DMSO. (A) A selection of MT intermediates as found frequently at early time points. Tubulin sheets formed by laterally associated, short oligomers (arrowheads) and short MT stumps (stars) can be seen. (B) Different time windows of MT assembly.