Incompetence of Neutrophils to Invasive Group A streptococcus Is Attributed to Induction of Plural Virulence Factors by Dysfunction of a Regulator
Figure 4
Severe invasive GAS degrade IL-8 by serine protease ScpC, resulting in impaired PMN migration.
(A) Migration abilities of PMN in response to IL-8 in the presence of non-invasive and invasive GAS, as listed in Figure 3A plus ScpC deficient NIH230 mutant (NIH230scpC). PMNs that migrated into the lower well in a transwell system were estimated by flow cytometry. (B). IL-8 was added into the culture medium (100 nM at a final concentration) and incubated with invasive or non-invasive GAS as listed in (A). Sixty minutes after incubation, the amount of IL-8 in triplicates was measured by sandwich ELISA and presented as the mean±SD. The results represent one of two independent experiments.