The Outcome of Phagocytic Cell Division with Infectious Cargo Depends on Single Phagosome Formation
Figure 3
Comparison of experimental and theoretical post-mitotic particle distribution.
(A) Post-mitotic DI of intracellular particle distribution. DI for C. neoformans strains 24067, H99, heat-killed H99 (HK H99), H99 phospolipase mutant (H99 PLB1), Cap67, heat-killed Cap67 (HK Cap67), Cap67 coated with polysaccharide (Cap67+PS), C. gattii or heat-killed C. gattii (HK C. gattii). The horizontal lines denoted the means of calculated DI, which were not immediately comparable between the groups as described in the Results. The data were collected from three or more independent experiments for every condition. In every experiment, movies were recorded for approximately 24 h with 300–400 cells in the microscopic field from which the macrophage-like cells underwent cell division with intracellular particles were selected for analysis. (B) Normalized post-mitotic DI by stochastic modeling of intracellular particle distribution. To eliminate the inaccuracy introduced by numerical variances of intracellular particles, the normalized DI was calculated as described in the Results. The distribution of intracellular particles was stochastic if the normalized DI equaled to one (dash line). While normalized DI were either larger or smaller than 1, the distribution of intracellular particles into daughter macrophage-like cells was non-stochastic and was skewed to unequal distribution or equal distribution, respectively. One-way ANOVA test revealed significant variances between the groups (p<0.0001). Newman-Keuls multiple comparison test revealed significant difference between H99 and H99 PLB1 (# p<0.05), Cap67 and Cap67+PS (## p<0.01). One sample t test revealed that post-mitotic distribution of most yeasts had significantly larger DI than 1 which denoted stochastic distribution (dash line) (**p<0.01, ***p<0.001).