Wingless Signalling Alters the Levels, Subcellular Distribution and Dynamics of Armadillo and E-Cadherin in Third Instar Larval Wing Imaginal Discs
Figure 12
Summary of quantification of changes in levels upon Armadillo mutant overexpression.
Levels of endogenous Armadillo (blue), overexpressed mutant (pink) and E-Cadherin (green) were assessed in (adherens) junctional, “cellular”, basolateral (membrane) and nuclear compartments. The “cellular” compartment assesses levels across a field of cells encompassing the basolateral membrane, nucleus and cytoplasm. Mutant constructs that were assessed are illustrated to the left of the graphs, and included ArmS10, ΔNArm1–128, ΔNArm1–155, Myr-ΔNArm1–155 and ArmΔCXM19. +1 and −1 represent maximal proportion changes in levels above and below the wild type baseline value of 0. Empty boxes indicate no change from wild type. dppGAL4 driver levels are lower in “lateral” relative to “central” domains of expression, as reflected by spotted versus solid colours. E-Cadherin levels were not assessed in the nuclear compartment (N/A).