In Vitro Analysis of Integrated Global High-Resolution DNA Methylation Profiling with Genomic Imbalance and Gene Expression in Osteosarcoma
Figure 4
Validation of Me-DIP-chip data using quantitative DNA methylation analysis.
Six genes from Table 1 were subject to EpiTYPER quantitative analysis of DNA methylation in CpG dinucleotides across 400 nucleotide regions detected as significantly enriched/depleted in Me-DIP-chip experiment. On left, the bar charts show levels of methylation (0–1–0–100%), on y-axis and individual CpG dinucleotides on the x-axis, and the corresponding error bars based on triplicate experiment. On right, the PGS-generated region views of the corresponding significantly enriched/depleted genes are labelled as in Figure 3.