The C/H3 Domain of p300 Is Required to Protect VRK1 and VRK2 from their Downregulation Induced by p53
Figure 8
p53 also induces downregulation of VRK2 isoforms that is protected by p300 and is sensitive to chloroquine.
(A). Effect of p300 on VRK2A or VRK2B downregulation. H1299 cells were transfected with pCB6+p53 (0.2 µg) and 5 µg of pCEFL-HA-VRK2A or pCEFL-HA-VRK2B plasmids. p300 was expressed from increasing amounts (µg) of plasmid. VRK2 and p300 were detected with an anti-HA antibody. The quantification of normalized levels of VRK2A and VRK2B are shown at the bottom. (B). Effect of chloroquine, an inhibitor of the endosome-lysosome vesicle transport. Cells were transfected with plasmids as indicated. The quantification of normalized levels of VRK2A and VRK2B are shown at the bottom.