Gating at the Mouth of the Acetylcholine Receptor Channel: Energetic Consequences of Mutations in the αM2-Cap
Figure 3
In each rate-equilibrium free-energy relationship (REFER) (residues I260–S268; 18′–26′), a point is the average of one mutant construct (Table S1). Φ is the slope of REFER. The Φ values are given in Table 2 and shown as a map in Fig. 5B. The agonist was either ACh (solid circles) or choline (open circles).