Pharmacological Inhibition of Nicotinamide Phosphoribosyltransferase/Visfatin Enzymatic Activity Identifies a New Inflammatory Pathway Linked to NAD
Figure 5
Inhibition of NAMPT enzymatic function with APO866 reduces intracellular NAD concentration and pro-inflammatory cytokine production in mouse and human inflammatory cells, without affecting viability.
(a) Mouse PEC and (b) human monocytes were cultured for 4 h with increasing doses of APO866, and then stimulated overnight with SAC or LPS, respectively. At the end of the culture, the culture supernatants were tested for TNFα, IL-1β and IL-6 content by ELISA, cell viability was assessed using the Live/Dead kit, and intracellular NAD concentration was determined as described in Methods. Data are mean ± SEM of triplicates. This panel is representative of at least 3 experiments performed.