Pharmacological Inhibition of Nicotinamide Phosphoribosyltransferase/Visfatin Enzymatic Activity Identifies a New Inflammatory Pathway Linked to NAD
Figure 1
Induction of NAMPT expression in collagen-induced arthritis.
Sera (a) and tissue extracts of paws (b) from CIA at day 14 (n = 8) and from non-arthritic, non-immunized, naïve (n = 7) mice were prepared and analyzed by NAMPT ELISA. *P<0.05 arthritic versus naïve in panel a and b. (c) NAMPT immunohistochemistry was performed on paw joints, using a specific rat anti-mouse NAMPT antibody (panels 1 and 2). Staining specificity was confirmed using an irrelevant isotype-matched antibody as primary antibody (panels 3 and 4). Synovial lining layer (SLL), synovial membrane (S) and pannus (P). Original magnifications: x100 for panels 1 and 3; x400 for panels 2 and 4.