Elevated Expression of Phospholipid Transfer Protein in Bone Marrow Derived Cells Causes Atherosclerosis
Figure 7
Characteristics of mouse macrophages.
A. Cholesterol efflux from cultured peritoneal macrophages. Cells were loaded with radioactive cholesterol as described in Methods. Subsequently, culture medium with human HDL as an acceptor was added. Left panel: Radioactivity was measured in aliquots from the cultured medium taken at 0, 1, 2, 4, and 6 hours. Circles: macrophages from C57BL/6J mice, squares: macrophages from huPLTPtg/tg mice. Differences were not statistically significant. B. PLTP activity measured in the medium of macrophages cultured for 24 hours in the absence (white bars) or presence (black bars) of 100 ng/mL LPS. Cells from 6 mice were used per group. C. PLTP activity measured in plasma samples from mice treated with thioglycolate. N = 6 mice per group. ** p<0.005, *** p<0.001 versus cells without LPS or thioglycolate (same genotype), ## p<0.005, ### p<0.001 versus PLPTwt/wt (same culture medium, with or without LPS or thioglycolate).