Elevated Expression of Phospholipid Transfer Protein in Bone Marrow Derived Cells Causes Atherosclerosis
Figure 6
Transplantations with β-actin GFP mice→LDLR−/− mice.
A–D: Early lesions (A,B) and advanced lesions (C,D) with donor cells expressing GFP (green), CD 68 (marker for macrophages) in red (A,C) and α-actin (marker for VSMCs) in red (B,D) and nuclei stained with DAPI (blue). Endothelial cells covering the lesions do not express GFP (nuclei indicated with arrows). Co-localization of GFP and CD68 results in an orange color (arrowheads). The necrotic core in the advanced lesion (located centrally in C and D) is diffusely positive for CD68 but does not show any GFP signal. The media is marked with a double arrow (↔). E,F: Early lesion (E) and advanced lesion (F) with donor cells expressing GFP (green), and CD 31 (marker for endothelial cells) in red (arrows). Representative pictures from N = 6 animals are shown. Original magnifications: 200X.