Generation of a Novel Regulatory NK Cell Subset from Peripheral Blood CD34+ Progenitors Promoted by Membrane-Bound IL-15
Figure 9
IL-10, IL-21 and HLA-G secreted by NKPSG cells act as immuno-regulatory factors.
(A) Effect of incubation with neutralizing anti-IL-10 mAbs (10 µg/ml) on the percentage of HLA-G positive human PB monocytes treated or not (untreated) with the cell supernantants from NKPSG cells. (B) Effects of the incubation with neutralizing anti-HLA-G mAbs (10 µg/ml) on the percentage of CD107a and IFN-γ on PB-derived NK positive cells triggered with NCR cross-linking in the presence or not of NKPSG cells. (C) Effects of the incubation with neutralizing anti-HLA-G mAb and/or soluble IL-21R (10 µg/ml) on the expression intensity (Mean Fluorescence Intensity) of HLA-DR, CD83, CD86 and mb-TGF-β on LPS-activated DC cultured or not with cell supernantants from NKPSG cells.