Generation of a Novel Regulatory NK Cell Subset from Peripheral Blood CD34+ Progenitors Promoted by Membrane-Bound IL-15
Figure 2
Autonomous NK commitment of PB-HP.
Commitment into the NK pathway of PB-HP was evaluated by flow cytometry at different times in culture, analyzing CD34, CD38 and the NK markers CD56, CD16, CD161, CD94, NKp30, NKp44, NKp46, NKG2D, CD69 and CCR7. Shadowed peaks represent staining with isotype control Abs. Continuous lines represent staining with the indicated Abs. The frequency of PB-HP represents about 0.1% of mononuclear cells of the peripheral blood. The data are representative of more than 30 separate experiments.