Engineering the Melanocortin-4 Receptor to Control Constitutive and Ligand-Mediated Gs Signaling In Vivo
Figure 4
A, Basal activity of wild-type MC4R, Rm1 (L106P), Rm2 (D122A), and mock-transfected cells were measured by detecting basal cAMP release and cAMP release in response to AGRP. B, Ratios of cAMP accumulation to cell-surface expression for wild-type MC4R, Rm1, and Rm2 were determined in the same batch of transiently transfected cells.
Values are means±s.e.m. of three independent experiments, each performed in quadruplicate.