Morphogenesis in Trypanosoma cruzi epimastigotes proceeds via a highly asymmetric cell division
Fig 8
TcPLK and TcKLIF localize to the FAZ and the posterior punctum during cell division.
(A) Cells carrying a 3xTy1-mNeonGreen::TcPLK allele were fixed and labeled with 1B41 to label the FAZ and posterior punctum (1B41, magenta), anti-Ty1 to label TcPLK (TcPLK, green), and DAPI to label DNA (DNA, blue). The cells were then imaged using fluorescence and DIC microscopy. Asterisks identify the 1B41 posterior punctum, empty arrowheads show TcPLK recruitment to the cell posterior, and the filled arrowhead shows TcPLK localization to the basal body. (B) Cells carrying a 3xTy1-mNeonGreen::TcKLIF allele were fixed and labeled with 1B41 to label the FAZ and posterior punctum (1B41, magenta), anti-Ty1 to label TcKLIF (TcKLIF, green), and DAPI to label DNA (DNA, blue). Asterisks show the 1B41 posterior punctum, empty arrowheads show TcKLIF location to the cell posterior, filled arrowheads show TcKLIF localization to the FAZ, and arrows show TcKLIF localization to the ingressing furrow.