A recombinant rabies virus chimera expressing the DC-targeting molecular MAB2560 shows enhanced vaccine immunogenicity through activation of dendritic cells
Fig 8
rCVS11-MAB2560 induced good immune memory after immunization in mice.
At the 3rd and 7th weeks following initial immunization, spleens were collected from the vaccinated BALB/c mice. Single cell suspensions of splenocyte cells (106 cells/mL) were stained with antibodies against TCM, T and B cell activation markers, and were analyzed with flow cytometry. (A) Flow gate strategy of CD4+ TCM (7th week post immunization). (B) The representative flow cytometric plots (7th week post immunization) and percentages of CD4+, CD44+ and CD62L+ recruited and/or activated CD4+ T cells in the spleens of immunized mice. (C) The representative flow cytometric plots (7th week post immunization) and percentages of CD4+, CD69+ recruited and/or activated CD4+ T cells in the spleens of immunized mice. (D) The representative flow cytometric plots (3rd week post immunization) and percentages of CD19+ and CD69+ recruited and/or activated B cells in the spleens of immunized mice. The data were presented as the means ± SD for each group. ns, not significant; *, P <0.05; **, P<0.01.