Treatment with benznidazole and pentoxifylline regulates microRNA transcriptomic profile in a murine model of Chagas chronic cardiomyopathy
Fig 2
Overview and characterization of immunoarray analysis.
(A) Schematic design of array workflow. Murine cardiac tissues were harvested 30 days after therapy and half of the heart was subjected to total RNA extraction, reverse transcription, and RT-qPCR of 752 miRNAs. Created with Biorender. (B) Heatmap and hierarchical clustering. The color scale illustrates the gene fold change after global normalization (row Z-score). Clustering was performed using the average linkage distance measurement method: Spearman Rank correlation. (C) Number of altered genes in each group. (D) Venn diagram showing the number of altered expressed genes in each group.